Novel indium-111 labeled gastrin peptide analogues (MG-CL1-4): synthesis and quality control.
Identifieur interne : 000509 ( Main/Exploration ); précédent : 000508; suivant : 000510Novel indium-111 labeled gastrin peptide analogues (MG-CL1-4): synthesis and quality control.
Auteurs : RBID : pubmed:23455200English descriptors
- KwdEn :
- MESH :
- chemical , blood : Gastrins.
- chemical , chemical synthesis : Gastrins.
- chemical , standards : Gastrins.
- standards : Isotope Labeling.
- Humans, Hydrogen-Ion Concentration, Indium Radioisotopes, Oxidation-Reduction, Protein Stability, Quality Control, Temperature, Time Factors.
Abstract
Radiolabeled neuropeptides are widely investigated to diagnose and therapy of tumors. These peptides get internalization after binding with particular receptors at the surface of cells and finally move to lysosome. Internalization into tumor cells helps in mapping the infected site. Minigastrin peptide analogues (MG-CL1-4) were synthesised and labeled with 111-In radioisotope under different sets of conditions for imaging CCk-2 receptor bearing tumors. Different parameters such as temperature (80-100°C), pH (4-12), incubation time (5-30 minutes) and dilution effect were investigated to get the maximum labeling yield and stability. The results indicated that MG-CL1-4 is successfully labeled with indium-111 at pH 4.5 with heating at 98°C for 15 minute. At these conditions i.e. heating, pH and incubation minimum oxidized and maximum labeling yield, more than 94 %, was obtained. The labeling stability was studied by incubating the radiolabeled complex for predefined time points in PBSA and blood serum. Results show that more than 90% radiolabeled MG-CL1-4 remained intact.
PubMed: 23455200
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Novel indium-111 labeled gastrin peptide analogues (MG-CL1-4): synthesis and quality control.</title><author><name sortKey="Naqvi, Syed Ali Raza" uniqKey="Naqvi S">Syed Ali-Raza Naqvi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, Government College University, Faisalabad, Pakistan.</nlm:affiliation><country xml:lang="fr">Pakistan</country><wicri:regionArea>Department of Chemistry, Government College University, Faisalabad</wicri:regionArea></affiliation></author><author><name sortKey="Khan, Zulfiqar Ali" uniqKey="Khan Z">Zulfiqar Ali Khan</name></author><author><name sortKey="Nagra, Saeed Ahmad" uniqKey="Nagra S">Saeed Ahmad Nagra</name></author><author><name sortKey="Yar, Muhammad" uniqKey="Yar M">Muhammad Yar</name></author><author><name sortKey="Sherazi, Tauqir A" uniqKey="Sherazi T">Tauqir A Sherazi</name></author><author><name sortKey="Shahzad, Sohail Shahzad" uniqKey="Shahzad S">Sohail Shahzad Shahzad</name></author><author><name sortKey="Shah, Syed Qaiser" uniqKey="Shah S">Syed Qaiser Shah</name></author><author><name sortKey="Mahmood, Nasir" uniqKey="Mahmood N">Nasir Mahmood</name></author><author><name sortKey="Ishfaq, Malik Muhammad" uniqKey="Ishfaq M">Malik Muhammad Ishfaq</name></author><author><name sortKey="Mather, Stephen John" uniqKey="Mather S">Stephen John Mather</name></author></titleStmt><publicationStmt><date when="2013">2013</date><idno type="RBID">pubmed:23455200</idno><idno type="pmid">23455200</idno><idno type="wicri:Area/Main/Corpus">000768</idno><idno type="wicri:Area/Main/Curation">000768</idno><idno type="wicri:Area/Main/Exploration">000509</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Gastrins (blood)</term><term>Gastrins (chemical synthesis)</term><term>Gastrins (standards)</term><term>Humans</term><term>Hydrogen-Ion Concentration</term><term>Indium Radioisotopes</term><term>Isotope Labeling (standards)</term><term>Oxidation-Reduction</term><term>Protein Stability</term><term>Quality Control</term><term>Temperature</term><term>Time Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Gastrins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Gastrins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="standards" xml:lang="en"><term>Gastrins</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Isotope Labeling</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Hydrogen-Ion Concentration</term><term>Indium Radioisotopes</term><term>Oxidation-Reduction</term><term>Protein Stability</term><term>Quality Control</term><term>Temperature</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Radiolabeled neuropeptides are widely investigated to diagnose and therapy of tumors. These peptides get internalization after binding with particular receptors at the surface of cells and finally move to lysosome. Internalization into tumor cells helps in mapping the infected site. Minigastrin peptide analogues (MG-CL1-4) were synthesised and labeled with 111-In radioisotope under different sets of conditions for imaging CCk-2 receptor bearing tumors. Different parameters such as temperature (80-100°C), pH (4-12), incubation time (5-30 minutes) and dilution effect were investigated to get the maximum labeling yield and stability. The results indicated that MG-CL1-4 is successfully labeled with indium-111 at pH 4.5 with heating at 98°C for 15 minute. At these conditions i.e. heating, pH and incubation minimum oxidized and maximum labeling yield, more than 94 %, was obtained. The labeling stability was studied by incubating the radiolabeled complex for predefined time points in PBSA and blood serum. Results show that more than 90% radiolabeled MG-CL1-4 remained intact.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">23455200</PMID><DateCreated><Year>2013</Year><Month>03</Month><Day>04</Day></DateCreated><DateCompleted><Year>2013</Year><Month>06</Month><Day>18</Day></DateCompleted><Article PubModel="Print"><Journal><ISSN IssnType="Print">1011-601X</ISSN><JournalIssue CitedMedium="Print"><Volume>26</Volume><Issue>2</Issue><PubDate><Year>2013</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Pakistan journal of pharmaceutical sciences</Title><ISOAbbreviation>Pak J Pharm Sci</ISOAbbreviation></Journal><ArticleTitle>Novel indium-111 labeled gastrin peptide analogues (MG-CL1-4): synthesis and quality control.</ArticleTitle><Pagination><MedlinePgn>299-305</MedlinePgn></Pagination><Abstract><AbstractText>Radiolabeled neuropeptides are widely investigated to diagnose and therapy of tumors. These peptides get internalization after binding with particular receptors at the surface of cells and finally move to lysosome. Internalization into tumor cells helps in mapping the infected site. Minigastrin peptide analogues (MG-CL1-4) were synthesised and labeled with 111-In radioisotope under different sets of conditions for imaging CCk-2 receptor bearing tumors. Different parameters such as temperature (80-100°C), pH (4-12), incubation time (5-30 minutes) and dilution effect were investigated to get the maximum labeling yield and stability. The results indicated that MG-CL1-4 is successfully labeled with indium-111 at pH 4.5 with heating at 98°C for 15 minute. At these conditions i.e. heating, pH and incubation minimum oxidized and maximum labeling yield, more than 94 %, was obtained. The labeling stability was studied by incubating the radiolabeled complex for predefined time points in PBSA and blood serum. Results show that more than 90% radiolabeled MG-CL1-4 remained intact.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Naqvi</LastName><ForeName>Syed Ali-Raza</ForeName><Initials>SA</Initials><Affiliation>Department of Chemistry, Government College University, Faisalabad, Pakistan.</Affiliation></Author><Author ValidYN="Y"><LastName>Khan</LastName><ForeName>Zulfiqar Ali</ForeName><Initials>ZA</Initials></Author><Author ValidYN="Y"><LastName>Nagra</LastName><ForeName>Saeed Ahmad</ForeName><Initials>SA</Initials></Author><Author ValidYN="Y"><LastName>Yar</LastName><ForeName>Muhammad</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Sherazi</LastName><ForeName>Tauqir A</ForeName><Initials>TA</Initials></Author><Author ValidYN="Y"><LastName>Shahzad</LastName><ForeName>Sohail Shahzad</ForeName><Initials>SS</Initials></Author><Author ValidYN="Y"><LastName>Shah</LastName><ForeName>Syed Qaiser</ForeName><Initials>SQ</Initials></Author><Author ValidYN="Y"><LastName>Mahmood</LastName><ForeName>Nasir</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Ishfaq</LastName><ForeName>Malik Muhammad</ForeName><Initials>MM</Initials></Author><Author ValidYN="Y"><LastName>Mather</LastName><ForeName>Stephen John</ForeName><Initials>SJ</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><Agency>Cancer Research UK</Agency><Country>United Kingdom</Country></Grant></GrantList><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Pakistan</Country><MedlineTA>Pak J Pharm Sci</MedlineTA><NlmUniqueID>9426356</NlmUniqueID><ISSNLinking>1011-601X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Gastrins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Indium Radioisotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>60748-07-4</RegistryNumber><NameOfSubstance>minigastrin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Gastrins</DescriptorName><QualifierName MajorTopicYN="N">blood</QualifierName><QualifierName MajorTopicYN="Y">chemical synthesis</QualifierName><QualifierName MajorTopicYN="N">standards</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Indium Radioisotopes</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Isotope Labeling</DescriptorName><QualifierName MajorTopicYN="N">standards</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Protein Stability</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Quality Control</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>6</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23455200</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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